Aim:

Different species of the genus Vibrio are pathogenic for humans, the aim of this study was to standardize a multiplex PCR to identify four Vibrio species.

Methods.

126 isolates environmental and 66 from the surveillance program INS were analyzed by PCR species-specific genes.

Results.

Of the total isolates, 124 were PCR positive for one of the species-specific genes. The PCR achieved the identification of the four target species (V. parahaemolyticus, V. alginolyticus, V. vulnificus and V. fluvialis), showing specificity and sensitivity between 1-2 ng/ul.

Discussion.

A multiplex PCR was developed that could provide reliable identification of four Vibrio species.

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