Abstract

DE S. SABINO, PAULO HENRIQUE et al. A method for measuring the concentration of CO₂ released by entomopathogenic nematodes. Rev. Colomb. Entomol. [online]. 2015, vol.41, n.2, pp.280-282. ISSN 0120-0488.

This study aimed to standardize a method for measuring the concentration of CO₂ released by infective juveniles (IJ's), in order to assess their metabolic activity. The nematodes used in this experiment were Heterorhabditis amazonensis JPM4 and Steinernema carpocapsae All. A standardized gas chromatography (GC) method was used for CO₂ analysis. There was a linear increase in CO₂ concentration associated with increased numbers of IJs. Additionally, the CO₂ concentration obtained for S. carpocapsae was higher than that obtained for H. amazonensis. The standardized GC method was adequate to measure the concentration of CO₂ released by IJ's.

Keywords : Metabolism; Standardization; Steinernematidae; Heterorhabditidae.

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