Abstract

RODRIGUEZ MARTINEZ, Raúl. Use of fluorescence in situ hybridization technique to visualize microorganisms. Rev. Univ. Ind. Santander. Salud [online]. 2011, vol.43, n.3, pp.307-316. ISSN 0121-0807.

Fluorescence in situ hybridization (FISH), is a technique that uses oligonucleotides probes labeled with fluorochromes which are directed to specific sequences of ribosomal ribonucleic acid (rRNA), this allows the rapid and specific identification of microbial cells whether as individual cells or grouped cells in their natural environment. Knowledge of the composition and distribution of microorganisms in natural habitats provides a solid support to understand interaction between different species in the microhabitat. This review shows how hybridization has evolved, the use of rRNA as target molecule, the type of labeling, the labeled uses today in fluorescent and the methodology, as well as the improvements that have been made to the FISH technique when is used in conjunction with other techniques in microbial identification. Salud UIS 2011; 43 (3): 307-316

Keywords : FISH; oligonucleotides; probes; rRNA; microbial diagnostic; fluorochromes; hybridization.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )