Abstract

GOMEZ KOSKY, Rafael et al. New method for rapid selection of banana (Musa spp. AAA cv. Grande naine) plants with bar gene in the field using ammonium gluphosinate. Rev. colomb. biotecnol [online]. 2010, vol.12, n.2, pp.248-258. ISSN 0123-3475.

Selector marker genes are widely used for the efficient transformation of different crops; however, there are very few references on the development of early selection methods in the fields of potentially transformed plants for herbicides. The objective of this research was to evaluate the effect of ammonium gluphosinate from the herbicide Finale® in leaf fragments from in vitro plants grown in the fields for the selection of putative transformed lines of banana cv. Grande naine (Musa spp. AAA), with a construction that has the bar gene as the selector marker gene. Since the method developed with the use of leaf fragments from field plants culture in vitro in the agar at 1% more water and 30.0 gl^-1 ammonium gluphosinate did differentiate the two transformed lines untransformed control, from the bar gene expression. Both lines were positive in the PCR molecular analysis. These results were a useful tool and form a part of a protocol of genetic transformation for banana, being the first reported result.

Keywords : Musa; genetic transformation; bar gene; ammonium gluphosinate.

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