Abstract

HERNANDEZ, María Andrea et al. The genomic identification of Colombian Acinetobacter baumannii clinical isolates by RFLP-PCR analysis of the 16S-23S rRNA gene spacer region. Rev. colomb. biotecnol [online]. 2011, vol.13, n.1, pp.110-114. ISSN 0123-3475.

The 16S-23S rRNA gene intergenic spacer (ITS) was analysed by RFLP in this study to identify A. baumannii from 139 isolates from four hospitals (identified as A, B, C and D). One hundred and twenty of these isolates (86.3%) belonged to the A. baumannii species; those identified as being A. baumannii were found to be polyclonal (19 clone groups) when determining the genetic relationships, 16 of them being found in hospital C. Hospitals A, B and D shared two clone groups isolated during different years. This study describes a rapid and easy method for genospecies identification of Acinetobacter baumannii.

Keywords : Acinetobacter baumannii-Acinetobacter calcoaceticus complex; 16S-23S rRNA gene intergenic spacer (ITS); RFLP-PCR..