Abstract

HERNANDEZ RENDON, César Augusto; SALAZAR MARIN, Yesica  and  RESTREPO BETANCUR, Luis Fernando. Embryos rescue for the obtaining of grapevine (Vitis vinifera L.) vitroplants. Rev. colomb. biotecnol [online]. 2013, vol.15, n.2, pp.193-201. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v15n2.41838.

This work is the first phase of a macroproyect about the optimization of a protocol for the obtaining of secondary metabolites of commercial interest by means of the use of cellular suspensions of Vitis vinífera L. The embryos rescue was investigated as alternative for the obtaining of grapevine (Vitis vinífera L.) vitroplants. The vegetable material used was obtained from commercial fruits of grapevine Red Globe variety. The seeds were disinfected submerging them in 5 g/l of dicloroisocianuric acid (NaDCC) for 15 min, and then in Benomyl^® 2 g/l for 15 min, with an effectiveness of 92%. Different treatments were performed to obtain plants using seeds as explants, which were cultured on Murashige Skoog medium supplemented with different concentrations of indole acetic acid (AIA) in combination with gibberellic acid (AG3) and kinetin (K) without obtaining favorable answer for the germination. As alternative, immature seeds were extracted from grape fruits and placed on Murashige Skoog medium supplemented with 100 mg/l polyvinyl pyrrolidone (PVP), 0.35 mg/l of gibberellic acid and 1.75 mg/l of indol acetic acid for a month. Seeds were then opened and performed embryo rescue, planting them under dark conditions for eight days in the culture media Murashige Skoog 1 and 2 modified, finding plants formation by 40% a month after growing.

Keywords : in vitro culture; growth regulators; dichloroisocyanuric acid; Red Globe.

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