Abstract

GONZALEZ NAVARRO, Bárbara O et al. First foray into the production of specific pathogen-free guinea pigs in Cuba. Rev. colomb. biotecnol [online]. 2014, vol.16, n.1, pp.93-98. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n1.44231.

Confidence and economics results are only obtained when the animals are isolated of the environmental and biological factors, that could interfere the course of from investigation and when is being established in them, a normal microflora balanced far from the presence of pathogen microorganisms. They were accomplished 26 histerectomies. The animals were fed with a modified formula of L-477 in the form of porridge until 21 days of age and permanently after with the C-484 solid and granulated, sterilized at 121^oC/20 minutes or 1,5Mrad. The forage or hay was consumed by the first generation since the 6 months of age. The gastrointestinal flora was administered by oral route with 0,5 ml of a dilution of 10^-6/g of the contents of the ileum final portion, cecum and first part of the colon of conventionalized Dunkin Hartley guinea pigs, at 24 and 48 hours born. It was used for microbiological bacteria monitoring Thioglycolate broth, Tryptic Soy broth and Sabouraud broth incubated aerobically at temperatures of 55, 37 and 25^oC respectively. There were obtained 51 newborns. The highest mortality was registered during the first 10 days of age (58,8%). There were archieved 12 animals: 9 female and 3 males. 6 of the female were reproduced approximately at 9 months of age and were archieved 11 normal delivery sucklings. There were obtained guinea pigs free of the followings specific pathogens: Salmonella sp., Pasteurella sp., Streptococcus of the group A y C. Bordetella bronchiseptica, Toxoplasma gondii, Sendai Virus, beside internal and external parasites.

Keywords : guinea pigs; specific pathogen free; isolators; hysterectomy and sterile diets.

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