Abstract

GABRIEL, Julio et al. Associations of molecular markers with diseases resistance, morphological and agronomic characters in potato diploid families (Solanum tuberosum L.). Rev. colomb. biotecnol [online]. 2016, vol.18, n.1, pp.17-32. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v18n1.57712.

Fefteen families of potato (824 genotypes) originated from inter-specific crosses among Solanum stenotomum, S. goniocalyx and S. phureja were genotyped in order to associate six molecular markers (GP94, HC, Nl25, Gro 1-4, RYSC3 y CP60). These molecular markers are linked to major genes for resistance to late blight (Phytophthora infestans), wart (Synchytrium endobioticum), root cyst nematode (Globodera pallida and G. rostochiensis) and viruses PVY and PVX. Results showed that five of six molecular markers were polymorphic and primers amplified in more than 80 % of the families. Molecular marker RYSC3 that is linked to Rya_dg gene did not amplify in any family. All individuals in Family 8 scored positive for three markers (CP60, GP94, and NL25). χ² test was used to determine the significance of the segregation ratios of each marker in every single family; so to the possible genotype of its parents was inferred. Nine morphological and agronomical characters were assessed during the harvest. Cluster analysis classified and selected 107 genotypes by putative resistance to PVX, P. infestans, G. rostochiensis and S. endobioticum, high yield, tuber volume and tuber number and shallow eyes. According to our results, practical aspects of efficient use of marker assisted selection in potato are discussed in this paper.

Keywords : Late blight; wart; virus; nematodes; polymorphic; major genes.

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