Abstract

VACA-VACA, Juan Carlos; CARRASCO-LOZANO, Emerson Clovis  and  LOPEZ-LOPEZ, Karina. Evaluation of the biotechnological potential of a promoter derived from passion fruit leaf distortion virus (PLDV), a begomovirus that infects passionfruit. Rev. colomb. biotecnol [online]. 2019, vol.21, n.1, pp.91-100. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v21n1.77636.

Biotechnological advances in plants require the bioprospecting of new promoters for the gene´s expression of agronomic interest, in particular, it is necessary to characterize new promoters with tissue-specific expression The objective of this research was to evaluate the expression activity of the AV1 gene promoter that codes for the capsid protein (CP) of the Passion fruit leaf distortion virus (PLDV) by means of transient tests of low pressure biobalistics. An analysis of the promoter region was carried out using bioinformatics tools. A CP-PLDV-GUS translational fusion was constructed, which carries the promoter region of the AV1 gene of PLDV fused to the uidA reporter gene (GUS). CP-PLDV-GUS was bombarded on leaves of tobacco seedlings grown in vitro using a gene gun. As a positive control pBI121 carrying the GUS gene under the control of the 35S promoter of CaMV was used. It was carried out 11 repetitions where the experimental unit was the leaf and the response variable the transient expression of the GUS gene represented by number of blue dots observed in the bombarded leaves. As a result, the non-parametric statistical analysis showed that there is sufficient sample evidence to confirm that both the AV1 promoter of PLDV and 35S of CaMV exhibit similar expression activity. Finally, the promoter of the AV1 gene of PLDV showed a strong activity of expression of the reporter gene in the leaf mesophyll cells, which could be used to confer tissue-specific expression in transgenic plants.

Keywords : Biobalistic; mesophyll; transient expression; uidA gene.

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