Abstract

BEDOYA VELEZ, Jessica María; CASTANO, Gustavo  and  OCHOA AGUDELO, Susana. Tolerance lead of native pseudomonas spp. wastewater from Aburrá Valley. Rev. colomb. biotecnol [online]. 2019, vol.21, n.1, pp.135-143. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v21n1.65146.

Lead (Pb) is a heavy metal whose physicochemical characteristics make it a persistent environmental pollutant, bioaccumulable and highly toxic. Bioremediation emerges as an alternative with multiple advantages compared to conventional treatments to remove contaminants such as heavy metals from wastewater, based on the tolerance capacities and/or resistance mechanisms of microorganisms. In order to know the tolerance of Pseudomonas spp. to the lead present in contaminated waters, four native isolates were obtained, coming from wastewater collected in the north from Aburrá Valley River, which were characterized biochemically. Tolerance bioassay was carried out in different concentrations of lead by agar diffusion, as a screening test, and determination of the minimum inhibitory concentration, determining the viability of the microorganism in each exposure. There was no significant difference for lead tolerance between the isolates, identified biochemically as Pseudomonas aeruginosa, nor between the different concentrations of lead to which the isolates were exposed. From these results, we discuss about the mechanisms reported by different authors, by means of which Pseudomonas spp. can act in the presence of lead. In this way, it is concluded that the isolates of Pseudomonas spp. they present tolerance and/or resistance mechanisms up to 2500 mg/L in the presence of lead nitrate, as potential agents that can be integrated into processes that promote new bioremediation technologies.

Keywords : Environmental bioremediation; metals heavy; water pollutants; water microbiology (Source: DeCS).

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )