Abstract

CORTES, Liliana Jazmín et al. Comparison of "in vitro" culture of Toxoplasma gondii RH strain in Hep-2 and Vero cell lines. Univ. Salud [online]. 2013, vol.15, n.2, pp.209-217. ISSN 0124-7107.

Introduction: Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, which is an obligate intracellular parasite capable of replicate itself in all nucleated cells, allowing their isolation and maintenance in cell culture. Objective: To compare cultivation Toxoplasma gondii (RH) in Hep-2 cells and Vero employed to spread parasitic. Methods: The conditions were optimized for cultivation of Toxoplasma gondii (RH) in Vero and Hep-2 cells lines, 500.000 and 1.000.000cel./mm³ were seeded and 1.000.000 and 2.000.000taq/mm³ were infected respectively, a track to determine cell invasion, percentage of rosettes and viability and efficiency of tachyzoites was made until the 120 hours. Results: After 48 hours of incubation, 90% of parasite invasion was evident in both cell lines as well as the largest count rosettes. At 120 hours higher counts of extracellular tachyzoites and a higher rate of performance of 185.9 in Hep-2 cells at concentration of 1.000.000taq/mm³ and 500.000cel./mm³ were obtained. Average parasite viability over 80 % was obtained for both cell lines. Conclusions: Higher parasitic performance was obtained viable in Hep-2 cells, so that this cell line can be considered as the preferred model for the propagation and maintenance of T. gondii (RH).

Keywords : Toxoplasmosis; culture medium; "in vitro"; cell line; performance test; cell survival.

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