Abstract

CARDENAS-BURGOS, Camilo Andrés; PACHECO-MALDONADO, José C  and  VANZELA, André L. L. In vitro Propagation of Solanum dolichosepalum (Solanaceae). Ciencia en Desarrollo [online]. 2016, vol.7, n.2, pp.9-22. ISSN 0121-7488.

Abstract A protocol for micropropagation of Solanum dolichosepalum was established from seeds disinfected with NaOCl and germinated in MS/4. Multiplication of microshoots was performed by nodal segments evaluating the effect of the nodal segment position, BA and IBA concentrations, MS salts concentration in presence of AIB, MS salts and sucrose concentration in the presence of activated charcoal, different culture media, and activated charcoal concentration on the total length of microshoots, average of newly formed buds, percentage of microshoots with basal callus and percentage of rooted microshoots. After 20 days of culture in MS/4 100% of cultured seeds developed plantlets with normal appearance. Microshoots multiplication was successful in medium H with 2 % sucrose supplemented with 0,02 mg L⁻¹ NAA and BA; after 30 days of subculture, the developed microshoots reached 4 cm in length, formed four axillary buds, 3% formed basal callus and 100% developed roots. Plantlets acclimatization was successfully performed on a mixture of soil, mulch and sand (3:2:1). This protocol is part of a program to produce grafted material of "Lulo de Castilla" for establishment of commercial orchards.

Keywords : Basal callus; Rooting; Lulo; Micropropagation; Wild solanaceae.

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