Abstract

RIVERA, Juan David; CORREA, Yaned Milena  and  OCAMPO, Diana Marcela. Standardization of a Chromatographic Method for the Identification of Giberelic Acid in Corn Seeds (Zea mays L.). Ciencia en Desarrollo [online]. 2017, vol.8, n.2, pp.51-60. ISSN 0121-7488.

Corn (Zea mays L.) is one of the most important cereal worldwide, because of its multiple industrial applications; besides, by having short germination times, it is useful in experimental processes. In the search of new alternatives that fast up seed germination processes, phytohormone stimulation is a mechanism that increasingly presents more studies in the literature in order to increase productivity in the agricultural sector in high consumption products such as son cereals. Inside these molecules, gibberellic acid is used in several ways to promote these processes. This is why it is necessary to implement analytic techniques that allow the identification and quantification of this phytohormone in a fast and efficient way. In this study, standardization of a method through high performance liquid chromatography with diode array detection for identification of giberellic acid in germinated corn seeds, was made. The best found conditions for the studied analyte were: stationary phase reversed, mobile phase in gradient of acetonitrile formic acid 0.01%, flux 0.5 mL min-1, running time 45.0 min. and analysis wavelength 195 nm. Under these parameters, gibberellic acid had retention time of 16.670 ± 0.050 min. These results allowed the proper identification of this hormone and it is considered that they could be adapted to determine this analyte in other arrays.

Keywords : Phytohormones; germination; HPLC; cereals.

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