Abstract

MARTINEZ, César A.; MOSQUERA, Oscar M.  and  NINO, Jaime. Apigenin glycoside: an antioxidant isolated from Alchornea coelophylla pax & k. Hoffm. (euphorbiaceae) leaf extract. Univ. Sci. [online]. 2016, vol.21, n.3, pp.247-257. ISSN 0122-7483.  https://doi.org/10.11144/Javeriana.SC21-3.agaa.

An antioxidant flavonoid has been isolated from methanolic leaf extract of Alchornea coelophylla Pax & K. Hoffm. by means of different column chromatography steps with DIAION HP-20 resin and silica gel in combination with analytical highperformance liquid chromatography (HPLC). It was identified as Apigenin-8-C- (α-L-rhamnopyranosyl-(1-›2)-β-D-glucopyranoside) on the basis of spectroscopic analysis and by comparison with related values reported in the literature. This compound exhibited high in vitro antioxidant activity through DPPH. and ABTS • + colorimetric assays with IC50 values of 7.528 and 379.7 µg. mL-1, respectively.

Keywords : ABTS ^{• +}; Column Chromatography; DPPH Euphorbiaceae; NMR; RP-HPLC..

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