Abstract

VANEGAS BERROUET, KATHERIN M.; GUTIERREZ SANCHEZ, PABLO A.  and  MARIN MONTOYA, MAURICIO A.. Molecular Identification of Fungi Isolated from Bean Tissues with Anthracnose Symptoms. Acta biol.Colomb. [online]. 2014, vol.19, n.2, pp.143-154. ISSN 0120-548X.  https://doi.org/10.15446/abc.v19n2.39154.

In this work, endophytic fungi from leaves and pods of bean presenting anthracnose symptoms were isolated from plants collected at different municipalities in the province of Antioquia (Colombia). Isolates were identified by sequencing the rDNA ITS regions together with the examination of reproductive structures during sporulation in culture media.Colletotrichum lindemuthianum, the causal agent of anthracnose was isolated in all samples showing symptoms of this disease. These results were confirmed by duplex PCR using the specific primers CD1/CD2 and CY1/CY2. Additionally, 17 endophytic fungi were obtained. Fourteen isolates did not sporulate in culture media (.Myceliasterilia) but were identified by phylogenetic analysis of the ITS regions as the Ascomycetes: .Leptosphaerulina (3), .Diaporthe (3), .Gibberella (1), .Plectosphaerella (1) and .Biscogniauxia (1) and the mitosporic genera Phoma (2), .Alternaria (2) and .Stemphylium (1). Three isolates were identified combining morphological and molecular analysis as Fusarium (2) and Curvularia lunata (1). This work increases our knowledge of the mycobiota of legume plants and will serve as support of future studies aimed at determining the effect of these fungi on the development of anthracnose as well as other problems affecting the bean crop.

Keywords : endophytes; PCR; Phaseolus vulgaris; rDNA.

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