Abstract

DIAZ M., Yessica Lorena; SERNA J, Johanna A.  and  TORRES V, Laura Sofía. Determination of conditions for protease encapsulation by electrospray. Rev. Cienc. Agr. [online]. 2016, vol.33, n.1, pp.03-08. ISSN 0120-0135.  https://doi.org/10.22267/rcia.163301.1.

Encapsulation is a method by which bioactive substances are introduced into a matrix to prevent their loss and also facilitate their incorporation into various products; notwithstanding, during this process, it is necessary to take into account technical and process conditions so as to achieve the formation of capsules. The objective of this research was to determine the appropriate conditions for protease encapsulation by electrospray. The process was evaluated according to the independent variables: voltage, concentration of coating material and feeding flow, in addition to dependent variables: spectral characteristics and morphology. In the obtained particles, morphological characteristics (microscopy) and specter (Raman) were measured. The results showed that the conditions allowing the encapsulation by electrospray were those performed at a voltage of 13kW, using a coating material with a contribution of total soluble solids 55%, and a feeding flow of 0.1mL/h, showing formation of spherical capsules with specters (Raman) in the range of 200 to 700 cm^-1, observing the highest peak, corresponding to the enzyme at 515cm^-1 and sizes between 0.035 and 1.185µm. From the results, is concluded that the electrospray may be considered as a viable technique for encapsulation of protease enzyme, provided that process conditions specific to each solution are established, thereby enabling the development of products (additives) which may be subsequently incorporated into other ones.

Keywords : Bioactive compounds; electrospinning; immobilization of enzymes; microencapsulation.

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