Abstract

CUARTAS O, PAOLA; VILLAMIZAR R, LAURA; ESPINEL C, CARLOS  and  COTES P, ALBA MARINA. Infection of native granulovirus on Tecia solanivora and Phthorimaea operculella (Lepidoptera: Gelechiidae). Rev. Colomb. Entomol. [online]. 2009, vol.35, n.2, pp.122-129. ISSN 0120-0488.

During 2004 and 2005, native strains of granulovirus obtained from Tecia solanivora larvae found in Cundinamarca, Nariño and Norte de Santander were isolated, identified and characterized. The effects of these infectious agents were studied on development of the potato moths T. solanivora and Phthorimaea operculella, evaluating variables such as width of the cephalic capsule, signs of viral infection and mortality. The effect of formulation was also determined on the efficiency of the different isolates formulated as a powder for potato seed protection. Additionally, the viral productivity of each isolate was studied using spectrophotometry. The highest viral production yields were obtained in P. operculella larvae, which were significantly higher than those obtained in T. solanivora larvae for all native isolates. The granulovirus infection in T. solanivora and P. operculella larvae caused a greater duration and overlap of the larval instars. The process of formulation did not significantly affect the efficacy of the granulovirus. Through a technique of weighted indicators, the isolate from Norte de Santander was selected as most promising due to its high efficiency and productivity.

Keywords : Baculovirus; Biological control; Viral infection.

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