Abstract

C. TORO, Esteban  and  A. GODOY, César. Effects of some reaction parameters on the production of ethyl esters using a combi-lipase of TLL and CALB in Lewatit VP OC 1600. Rev. ion [online]. 2020, vol.33, n.2, pp.83-97.  Epub Aug 30, 2020. ISSN 0120-100X.  https://doi.org/10.18273/revion.v33n2-2020007.

Optimization of mixtures of immobilized lipases (combi-lipases) has been applied as a strategy to increase the rate and the yield in the production of alkyl esters of fatty acids or in hydrolyzed vegetable oils compared to conventional biocatalysis (monoenzymatic catalysts). Previously, Combi-lipase (CL) of Thermomyces lanuginosus (TLL) and Candida antarctica isoform B (CALB) in the proportions 75:25 in the Lewatit VPOC 1600 (LW) support were identified as the most active with palm olein, from according to the amount of immobilized protein and its efficiency in the production of ethyl esters (EE) under mild reaction conditions. However, its stability under different conditions has not been studied. Thus, it was determined that this CL produces EE in a stable way under different temperatures and in the presence of water and free fatty acids, by contrast, the addition of glycerol generated decreases of up to 70% in the synthesis of ethyl esters. Applying the Arrhenius model for a pseudo-first order reaction, an Ea of 21.4 ± 1.4 kJ/mol was determined, meaning a decrease of 5% compared to its main component (TLL-LW). Additionally, the use of a different oil (soybean) generated a 20% increase in EE production. Finally, a docking study and the kinetic simulation of the system were performed using a previously developed model, but a clear correlation with the experimental results was not achieved. Apart from this, due to its robustness, the CL studied has a high potential for application in synthesis of ethyl esters.

Keywords : Biocatalysts; Combi-lipases; Palm Oil; Docking; Ethyl Esters; Enzymes.

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