Abstract

VIVAS-REYES, Ricardo; AHUMEDO, Maicol  and  CABEZAS, José. HOMOLOGY MODELLING BY THREE-DIMENSIONAL STRUCTURE OF PHOSPHOLIPASE A₂ CYTOSOL-DEPENDENT PANCREATIC CALCIUM IN Rattus norvegicus. Rev.Colomb.Quim. [online]. 2010, vol.39, n.2, pp.181-197. ISSN 0120-2804.

Calcium-dependent phospholipase A2 (cPLA₂) is of vital importance in biosynthesis of lipid, inflammatory or eicosanoid mediators, such as thromboxanes, leukotrienes and prostaglandines. For the 85 KDa cPLA₂ present in Rattus norvegicus, its primary structure (AA sequence) is well known, but there is no report of its secundary and tertiary structures. In this study tertiary structure models of cPLA2 present in pancreatic beta cells of rattus norvegicus were obtained and evaluated using protein modelling and evaluation via internet servers. The cPLA₂ 3D models obtained can be useful to the rational design of site-directed mutagenesis experiments, for elucidating and understand which might be the operation mechanism of this enzyme or for the design of drugs which may bind to it.

Keywords : modelling; phospholipase A2; bioinformatics.