Abstract

JURADO-GAMEZ, H; CALPA-YAMA, F  and  CHASPUENGAL-TULCAN, A. IN VITRO DETERMINATION OF THE PROBIOTIC Lactobacillus plantarum ACTION ON Yersinia pseudotuberculosis ISOLATED FROM Cavia porcellus. Rev. Med. Vet. Zoot. [online]. 2014, vol.61, n.3, pp.241-257. ISSN 0120-2952.  https://doi.org/10.15446/rfmvz.v61n3.46872.

The in vitro effect of Lactobacillus plantarum on Yersinia pseudotuberculosis isolated from guinea pig identified and evaluated biochemical and molecular. L. plantarum viability was determined at different pH (3.25, 4.5, 7.6 y 8.2), bovine bile salts (3, 4 y 5%), bovine bile (0.5, 1 y 2%) and temperature (38 y 45°C). Both bacteria susceptibility was performed with Cephalothin, Cefepime, Ciprofloxacin, Dicloxacillin, Enrofloxacin, Gentamicin, Trimethoprim-Sulfamethoxazole and Penicillin. L. plantarum peptides were determined by HPLC and growth kinetics on two substrates (MRS and Pro), measuring biomass, pH, total sugar consumption and lactic acid. Better growth was observed at 3.25 pH, 5% bile salts, 2% bovine bile and temperature 45 °C. with values 3,0·10¹², 2.3·10¹², 8.6·10¹¹ y 3.0·10¹² UFC/ml, respectively. L. plantarum and the supernatant inhibited Y. pseudotuberculosis, with halos of 3 to 5 mm. L. plantarum and resistance to penicillin and Y. pseudotuberculosis Dicloxacillin was observed, respectively, with sensitivity of both strains to other antibiotics. Peptides were evidenced VAL-TIR-VAL y TIR-GLI-GLI-FA-MET in the supernatant of L. plantarum. During the logarithmic phase for MRS and Pro 1012 values were reached 6.75·10¹² y 1.20·10¹² CFU / ml (biomass), 6.96 and 5.49 (pH) and 0.65 and 0.76 % (lactic acid) respectively. The comparison of means using a randomized block design (P > 0.05) showed similar behavior. L. plantarum demonstrated their probiotic potential in vitro conditions.

Keywords : Antibiotics; biomass; inoculum; functional.

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