Abstract

RODRIGUEZ MARTINEZ, Raúl  and  SUESCUN OTERO, Gina. Applications and inconvenient of Fluorescence in situ hybridization technique (FISH) in the identification of microorganism. Salud, Barranquilla [online]. 2013, vol.29, n.2, pp.327-340. ISSN 0120-5552.

During these recent years, a large number of FISH technique applications have been reported. These techniques have been used in the detection of microorganisms in their own habitat without requiring their previous isolation and purification. The importance of FISH lies in the ability of the DNA probe to detect a specific region of the nucleic acid of microbial cells and to be visualized by epifluorescence microscopy. This review describes the various FISH uses ranging from the identification of the microbiota in aquatic environments and their use in bioremediation, to the detection of pathogens in clinical diagnosis. It also presents some limitations as well as the potential solutions to be applied when the FISH technique is used.

Keywords : Hybridization; Fluorescence; Probe; FISH; Fluorochromes; Application.

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