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Revista Facultad de Odontología Universidad de Antioquia
Print version ISSN 0121-246X
Abstract
NACIF, MCAM; MARCELIANO-ALVES, MFV and ALVES, FRF. CONTAMINATION OF GUTTA-PERCHA CONES IN CLINICAL USE BY ENDODONTIC SPECIALISTS AND GENERAL PRACTITIONERS. Rev Fac Odontol Univ Antioq [online]. 2017, vol.28, n.2, pp.327-340. ISSN 0121-246X. https://doi.org/10.17533/udea.rfo.v28n2a6.
Introduction:
the present study evaluated the microbial contamination of gutta-percha cones proceeding from packages used clinically by endodontic specialists and general practitioners.
Methods:
two gutta-percha cones were selected from 30 original packages, already in clinical use, in dental clinics. The cones were transferred directly to test tubes containing thioglycolate broth and incubated at 37 °C for 21 days in aerobiosis. All tests were done in triplicate. Fractions proceeding from the tubes that presented turbidity were plated in CLED agar and Gram staining.
Results:
among the gutta-percha cone boxes tested, 9 (30%) showed bacterial contamination in the tested cones, 4 (13%) of those coming from general practitioners and 5 (17%) coming from specialists. There was no significant difference in the contamination of cones in relation to their origin (p>0,05).
Conclusion:
the results of the present study reinforce the need for both clinical dentists and endodontics specialists to implement a strict disinfection protocol before using gutta-percha cones, due to the frequency of contamination.
Keywords : bacterial contamination; endodontic infection; gutta-percha; endodontic treatment; root canal.