Abstract

VEJARANO-ORJUELA, Álvaro; HERNANDEZ-VASQUEZ, Aureliano; RONDON-BARRAGAN, Iang S.  and  SUAREZ-MESA, Rafael A.. Luteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cows. Orinoquia [online]. 2020, vol.24, n.1, pp.42-51. ISSN 0121-3709.  https://doi.org/10.22579/20112629.590.

The luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance.

Keywords : bovine creole reproduction; LHR-LHCGR; fertility indicator..

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