Abstract

MENDEZ BEJARANO, CLAUDIA PATRICIA et al. THE IMPRINT AS A NON-INVASIVE METHOD OF SAMPLING FOR THE MOLECULAR DIAGNOSIS OF CUTANEOUS LEISHMANIASIS IN COLOMBIA'S MILITARY POPULATION. Rev. Med [online]. 2018, vol.26, n.2, pp.15-21.  Epub Dec 01, 2018. ISSN 0121-5256.

Taking into account that the traditional methods of sampling and diagnosis for cutaneous leishmaniasis , have limitations, such as direct smear, whose sensitivity depends on the professional's expertise, the lesion aspiration that can be used to detect parasites in the lamina, DNA or cultures takes a long time and is demanding, and the biopsy of the lesion that is invasive and painful were compared with the imprinting method on the filter paper of the ulcerative lesion against the traditional method of aspiration by means of the conventional PCR technique using as a target a DNA region of the parasite kinetoplast. In this present work, PCR obtained an imprinting sensitivity of 90.07% compared to the aspirate of 86.3%, which, besides being a non-invasive sampling method, with few transport requirements, it can be taken directly in the area of operations at a very low cost, which turns out to be beneficial to be used in patients with cutaneous leishmaniasis of the National Army of Colombia, who are in different operations areas.

Keywords : cutaneous leishmaniasis; imprint; filter paper; PCR.

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