Abstract

ARBELAEZ, Gustavo et al. Improvement of two diagnostics methods for detection of influenza swine virus. Univ. Sci. [online]. 2008, vol.13, n.1, pp.65-74. ISSN 0122-7483.

The swine influenza virus, is an acute disease that affects the respiratory tract of the pig, It is highly contagious and have a raised morbility ratio also could turn into a fatal end. This disease has a very important roll into the pig production farms since it is a cause of loses of funds. For that reason, this work implemented the cell culture technique and RT-PCR for M, H and N proteins segments for the fast and accurate diagnosis of this virus. On this work, were tested 82 nasal swabs, 12 broncoalveolars washes, and 12 lung tissues, given negative results for the swine influenza virus. During the compilation of RT-PCR for M protein segment with the cell culture as a gold standard, it attained a sensibility and specificity of 100% and Kappa index of 1 indicating a good concordance between the two probes.

Keywords : hemagglutinin; neuroaminidase; influenza; MDCK y RT-PCR.