Abstract

VARGAS GARCIA, César Augusto; GARCIA SANCHEZ, Carlos Eduardo; ARGUELLO FUENTES, Henry  and  TORRES SAEZ, Rodrigo Gonzalo. Metabolic Flows Balance in Saccharomyces cerevisiae based on Intracellular Compartmentalization. Rev. colomb. biotecnol [online]. 2013, vol.15, n.2, pp.18-28. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v15n2.41263.

Flux Balance Analysis - FBA - is one of the most used techniques in prediction of microorganism bioproducts. It requires an objective function that represents biological objective of the studied microorganism. This paper presents a new kind of objective functions based on individual physical compartment objetives in the studied microorganism. These kind of functions was tested with a stoichiometric model extracted from iMM904 reconstruction of S. cerevisiae and its performance is compared with the most used objective function in literature, growth maximization, in anaerobic and aerobic batch conditions. The presented objective function outperform growth predictions in 10% and ethanol predictions in 75% compared with obtained by maximization of growth objective function, in anaerobic conditions. In aerobic batch conditions the presented objective function outperforms in 98% growth preditions and 70% ethanol predictions compared with growth maximization.

Keywords : Flux Balance Analysis; FBA; iMM904 reconstruction; Compartment based Objective Function.

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