Abstract

SANCHEZ CASTELBIANCO, Etna Milena; HEREDIA MARTIN, Juan Pablo; BUITRAGO MORALES, Sonia Marcela  and  MEDINA RODRIGUEZ, Juan Pablo. Isolation and identification of potential amylolytic and cellulolytic microorganisms from soil of Bogota wetlands. Rev. colomb. biotecnol [online]. 2020, vol.22, n.1, pp.36-44. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v22n1.71278.

The amylases and cellulases obtained from microorganisms have been used since more than three decades in industry. The isolation of native microbial strains with amylolytic and cellulolytic ability is the starting point to make the best of microbial biodiversity and support the production of amylases and cellulases with novel characteristics to obtain new products and optimize industrial processes where these enzymes can be applied. The objective of this work was to isolate microbial strains with the capacity to produce amylolytic and cellulolytic enzymes from the soil of five wetlands in Bogotá. Hydrolysis halos measurements in starch agar and carboxymethylcellulose agar were performed. The enzymatic activity was determined through the production of reducing sugars which were determined by 3,5-dinitrosalicylic acid method. Four different amylolytic isolations were selected and all of them were identified as Bacillus amyloliquefaciens. The amylolytic activity was between 480 ± 35 and 752±33 U/mL at 60°C. Five different cellulolytic strains were selected and two of them were identified as Bacillus amyloliquefaciens, two as Yersinia massiliensis and one as Stenotrophomonas nitritireducens. Their cellulolytic activities were from 13.82 ± 2.51 to 19.11 ± 2.3 U/mL at 50°C. These results demonstrate that as a part of the Bogota wetlands soil biodiversity there are microorganisms producing amylases and cellulases which might be applied in industrial processes.

Keywords : amylolytic activity; cellulolytic activity; Bacillus subtilis; Stenotrophomonas nitritireducens; Yersinia massiliensis.

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