Abstract

LAGOS-BURBANO, Tulio César; LAGOS-SANTANDER, Liz Katherine; DUARTE-ALVARADO, David Esteban  and  GARCIA-ALZATE, Javier. Induced mutagenesis in Solanum betaceum Cav. plants by the use of diethyl sulphate (DES). rev.udcaactual.divulg.cient. [online]. 2022, vol.25, n.1, e1956.  Epub May 19, 2022. ISSN 0123-4226.  https://doi.org/10.31910/rudca.v25.n1.2022.1956.

The raw material for plant breeding is genetic variability, which is low in species in the process of domestication that have not been subjected to selection, as is the case with Solanum betaceum. One of the technologies to increase genetic variability is mutagenesis induction. The objective was to evaluate, through RAMs markers, the molecular variations present in S. betaceum seedlings from seeds previously subjected to different concentrations of the mutant agent diethyl sulfate (DES). The polymorphic loci ranged from 87.5 to 100%, number of effective alleles (Ne) between 1.0 and 1.99. The most polymorphic loci were observed in TG, AG, ACA, and CGA, which showed a mean unbiased heterosis between 0.34 and 0.51 with an average of 0.44, which allows establishing that these markers are useful to obtain greater discrimination between mutants in S. betaceum. Genetic distances ranged from 0.30 to 1.0. The 81.28% of these records were between 0.60 and 0.90. This reveals a low level of changes due to DES. These small changes contribute to enriching the genetic variability of the DES-treated sample. The RAMs markers were useful for detecting changes between plants from DES treated seeds and normal plants. Genetic variability between DES treatments was higher than non-DES treatments. Genetic similarities were low between treated and untreated plants and were high among untreated plants. The changes produced by DES were of low magnitude, however, they produced changes in the levels of genetic variability.

Keywords : Mutagenesis; Polymorphic loci; Ramdom amplified microsatellite; Tree tomato; Unbiased heterozygosity.

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