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Infectio
Print version ISSN 0123-9392
Abstract
SANCHEZ, Lizeth Paola et al. Development and implementation of a multiplex PCR for the detection of 4 species of Vibrio. Infect. [online]. 2022, vol.26, n.3, pp.199-204. Epub Oct 09, 2023. ISSN 0123-9392. https://doi.org/10.22354/24223794.1047.
Aim:
Different species of the genus Vibrio are pathogenic for humans, the aim of this study was to standardize a multiplex PCR to identify four Vibrio species.
Methods.
126 isolates environmental and 66 from the surveillance program INS were analyzed by PCR species-specific genes.
Results.
Of the total isolates, 124 were PCR positive for one of the species-specific genes. The PCR achieved the identification of the four target species (V. parahaemolyticus, V. alginolyticus, V. vulnificus and V. fluvialis), showing specificity and sensitivity between 1-2 ng/ul.
Discussion.
A multiplex PCR was developed that could provide reliable identification of four Vibrio species.
Keywords : Vibrio infections; body of water; multiplex polymerase chain reaction; Vibrio.
