Abstract

MATULEVICH-PELAEZ, Javier-Andres; CASTRILLON-CARDONA, William-Fernando; RODRIGUEZ-RENUMA, Laura-Marcela  and  MEDINA-DONCEL, Juan-Pablo. Phytochemical Study and Evaluation of the Antioxidant Capacity of Senna spectabilis Flowers Obtained in the Colombian Andean Region. Rev. Cient. [online]. 2021, n.42, pp.315-327.  Epub Oct 20, 2021. ISSN 0124-2253.  https://doi.org/10.14483/23448350.17592.

The present work shows the results of the phytochemical study of Senna spectabilis flowers and the evaluation of their antioxidant capacity, for this, the plant material was collected in the municipality of Guaduas (Cundinamarca) and identified through the Colombian National Herbarium with the number collection COL 600165, after extraction processes by cold maceration, 100 g of ethanolic extract were obtained, which was fractionated with solvents of increasing polarity, obtaining fractions of heptane, dichloromethane and ethyl acetate, then they were subjected to separation processes by Thin layer chromatography (TLC) and column chromatography (CC), the tentative identification of the obtained mixtures and isolated compounds was carried out by gas chromatography coupled to mass spectrometry (GC-MS), allowing the identification of three mixtures made up of ester, ketone, anthraquinone, monoterpenoid and a derivative of cinnamic acid compounds; and the compounds methyl cinnamate and caffeine. The antioxidant capacity of the obtained fractions and the ethanolic extract were studied by the DPPH method, in addition, the measurement of total phenols and the quantification of flavonoids were carried out. The results indicate that the dichloromethane fraction exhibited an inhibition percentage greater than 30 % and that the highest concentration of phenols and flavonoids is found in this same fraction. In this way, the work gave a chemical contribution (isolation of methyl cinnamate and caffeine, and three mixtures) and a biological contribution after identifying the fraction of dichloromethane obtained from the flowers of the species as the one with the greatest amount of substances with antioxidant capacity. has respect to the other fractions and extracts.

Keywords : caffeine; phytochemistry; Senna spectabilis..

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