Abstract

JUAN-PABLO, HEREDIA-MARTÍN  and  ETNA-MILENA, SÁNCHEZ-CASTELBLANCO. Microorganism and substrate evaluation from organic wastes for cellulases production. Rev.Bio.Agro [online]. 2023, vol.21, n.2, pp.50-61.  Epub Dec 01, 2023. ISSN 1692-3561.  https://doi.org/10.18684/rbsaa.v21.n2.2023.2165.

The necessity of reducing enzyme production costs and their wide use in several industrial sectors, have increased the interest on agro-industrial wastes valorization as substrates for the obtention of these bioproducts. Given that agricultural wastes are both a source of cellulose and cellulolytic microorganisms, the purpose of this study was the selection of previously isolated cellulolytic bacteria from wastes generated in marketplaces and, the evaluation of media formulated from this waste. 11 bacterial strains were grown in Mandel’s broth for 72 hours evaluating their cellulolytic activity at 30 and 50 ºC. To formulate the culture media, the cellulose, nitrogen and total organic carbon content in corn cob leaves and legume seed pods collected in a marketplace was determined. Three types of media were made using cellulose, sourced from waste, supplemented with peptone, ammonium sulfate and urea: the last three added as Nitrogen source. Medium A was formulated with chopped and dehydrated wastes. Medium B had the same treatment than medium A plus a liquefy step. Medium C was made with un-dehydrated and liquefied waste. From the 5 selected cellulolytic strains, C6M2 was cultured at 37 ºC on each of the three media and the enzyme activity was monitored for 96 hours of fermentation. Cellulase production was achieved in media A and B. Medium A presented higher production (19,82 ± 3,0 U/mL). On the other hand, no cellulolytic activity was evidenced in medium C. The corn cob leaves and legume seed pods, chopped and dehydrated, allow the cellulase production using the bacterium strain C6M2.

Keywords : Cellulose; Cellulases; Agriculture wastes; Corncob leaves; legume seed pods; Marketplaces; Cellulolytic bacteria; Nitrogen; Carbon; Substrate; Culture media.

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