Abstract

AVILA PORTILLO, Luz Mabel et al. Comparison of the viability and growth in stem cell culture obtained from adipose tissue (ADAS) pre and post freeze. Nova [online]. 2015, vol.13, n.24, pp.27-38. ISSN 1794-2470.

Objective. Comparing viability, growth in culture, phenotype and osteogenic lineage differentiation of Adipose-Derived Adult Stem Cells (ADAS) pre and post freezing. Methods. Samples were obtained from adipose tissue of healthy individuals who underwent liposuction, to which underwent enzymatic digestion with collagenase I. The stromal vascular fraction (SVF) obtained was divided equally into two fractions one of which was cryopreserved and stored at -196 °C and another cultivated to passage 1 (P1) before being cryopreserved. Both fractions were cryopreserved by chamber automated freezing using two solutions: (A) DMSO/Dextran40/HSA and (B) DMSO/HESSICO/HSA kept 3 months at -196°C and thawed in PBS/HSA/HESSICO. After its thawing were also subjected to cultivation, count, cell viability, phenotyping and osteogenic potential. Results. Found that frozen cells with cryoprotectant solution DMSO/Dextran40/HSA and a pre-freezing culture, viability percentages exhibited higher and reached 80% confluence in less time. Expanded samples showed no differences in the phenotype by flow cytometry and shown to osteogenic lineage differentiation.

Keywords : mesenchymal stem cells; adipose tissue; cryopreservation.

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