Abstract

VACA-VACA, JUAN CARLOS; JARA-TEJADA, FRENYILINE  and  LOPEZ-LOPEZ, KARINA. Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia. rev.colomb.cienc.hortic. [online]. 2020, vol.14, n.1, pp.115-124.  Epub Apr 16, 2022. ISSN 2011-2173.  https://doi.org/10.17584/rcch.2020v14i1.10434.

Weeds are a source of new viruses but are often neglected during diversity studies. Eleven weed samples previously were collected along the edges of tomato crops in four municipalities (Florida, Ginebra, Cerrito and Candelaria) in the southeast of Valle del Cauca. These samples were positive for begomoviruses, but their molecular characterization had not been done until now. For each sample, DNA fragments were cloned, sequenced and analyzed. Nucleotide sequence analysis of viral fragments showed the presence of six different begomoviruses: two viruses isolated from Lantana camara, Desmodium sp. and Amaranthus dubius, which were previously identified as potato yellow mosaic virus (PYMV) and passionfruit leaf distortion virus (PLDV), respectively; four viruses isolated from L. camara, A. dubius, Rivina humilis, Desmodium sp., Rhynchosia minima, Hybanthus attenuatus, Verbena sp., Croton hirtus and Caesalpinia sp., showed the highest nucleotide sequence identity (89%) with the bean chlorotic mosaic virus (BChMV), datura leaf distortion virus (DaLDV) and rhynchosia golden mosaic Yucatan virus (RhGMYV). The cloned virus fragments isolated from these weeds could be new begomoviruses that have not been previously reported, as determined with the ICTV species demarcation criterion for the genus Begomovirus (≥91 % sequence identity). Mixed infections of begomoviruses in Desmodium sp. and A. dubius. Finally, this paper reports for the first time three alternative begomovirus weed hosts that infect tomato and passion fruit crops: A. dubius for PYMV, and L. camara and Desmodium sp. for PLDV, both viruses which infect tomato and passion fruit crops, respectively.

Keywords : geminivirus; hosts; Solanaceae; weed.

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