Abstract

ANGULO-FLOREZ, Diego Hernando; CASAS-MATEUS, Jaime Augusto; CIPAGAUTA-ESQUIVEL, Edna Carolina  and  APARICIO-PLAZAS, David Eduardo. Didactic intervention for the extraction and quantification of steroids in urine. Prax. Saber [online]. 2021, vol.12, n.31, pp.69-90.  Epub Apr 21, 2022. ISSN 2216-0159.  https://doi.org/10.19053/22160159.v12.n31.2021.11215.

This article describes a didactic strategy for teaching a miniaturized solid-phase extraction method in the context of laboratory practical training [LPT]. It is based on the students' previous imaginaries in order to generate conceptual changes and promote meaningful learning through a few laboratory sessions. This article will report on the experience of combining the use of a sample preparation technique-PT-SPE-with a didactic intervention exercise for the improvement of procedural skills. We present the results of the application of LPT in the multi-determination of three steroids in urine samples using HPLC-DAD-PT-C18-SPE, which show adequate recovery rates and validation parameters. The results of the intervention show improvement of skills related to solid-phase extraction methods. It is concluded that there were conceptual changes in analytical processes, an adequate familiarization with new sample preparation methodologies, a significant increase in chemical vocabulary, and understanding of concepts, along with the application of lower-order cognitive processes to the enhancement of higher-order cognitive skills.

Keywords : experimental science didactics; laboratory practical training; sample preparation; SPE; HPLC.

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