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Vitae
versión impresa ISSN 0121-4004
Resumen
OVIEDO-CASTRILLON, Alejandro; QUINTERO-LAVERDE, Natalia; VELASQUEZ-RODRIGUEZ, Claudia y LOPEZ-MARIN, Beatriz Estella. Comparison and validation of a fast method for the extraction and quantification of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids. Vitae [online]. 2016, vol.23, n.3, pp.194-201. ISSN 0121-4004. https://doi.org/10.17533/udea.vitae.v23n3a05.
Background:
Omega 3 family fatty acids are currently widely studied for the diverse effects they have on cardiovascular disease risk factors. Seafood is the main natural source of these compounds.
Objetives:
In this paper the accuracy is verified on the fat extraction in fish, between two methods, using one of the most employees as it is the Bligh & Dyer since 1959, compared with SOXTEC, one more updated method with more modern equipment and validated quantification method for some omega 3 fatty acids by gas chromatography.
Methods:
Fat extraction was performed by SOXTEC and Bligh & Dyer with the use of a Certified Reference Material (SRM 1946). After having assessed the accuracy of both systems, a quantification method of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids was validated through gas chromatography.
Results:
It was found that the method proposed by Bligh & Dyer in 1959 is the most accurate because it showed better recovery percentages. Also, it was found that the quantification validation method of the most important omega 3 fatty acids complies with every parameter assessed throughout the validation.
Conclusions:
In conclusion SOXTEC being a more modern method which uses the latest technology, the Bligh & Dyer is the most effective method for removing fat in fish and gas chromatography allows quantification of fatty acids with precision and accuracy as meets the evaluated parameters.
Palabras clave : Fatty acids; gas chromatography; Bligh & Dyer; EPA; DHA; omega 3.