Resumen

JARAMILLO MESA, Helena; MARIN MONTOYA, Mauricio  y  GUTIERREZ SANCHEZ, Pablo Andrés. Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passion fruit ( Passiflora edulis f. edulis ). Rev. Fac. Nac. Agron. Medellín [online]. 2019, vol.72, n.1, pp.8643-8654. ISSN 0304-2847.  https://doi.org/10.15446/rfnam.v72n1.69438.

Purple passion fruit (Passiflora edulis f. edulis), also known as gulupa, is a vine plant of the family Passifloraceae, which in recent years has gained importance in the world fruit market due to its exotic nature and excellent organoleptic properties. Although the demand for gulupa in Colombia has increased significantly to become one of the most important fruit exports, the cultivated area has been in decline since 2009 due to the impact of plant diseases. Cucumber mosaic virus (CMV), Soybean mosaic virus (SMV) and Cowpea aphid-borne mosaic virus (CABMV) are amongst the main viruses found infecting gulupa in Colombia. To further characterize the virome of gulupa, a deep sequencing transcriptome study was performed from a producing region in eastern Antioquia. Based on the results of next-generation sequencing (NGS), we report the genome sequence of a tymovirus infecting this plant. Phylogenetic analysis revealed this virus to be a close relative of Passion fruit yellow mosaic virus (PFYMV), Cassia yellow mosaic-associated virus (CYMaV) and Calopogonium yellow vein virus (CYVV). To date, only a 1,115 nt segment comprising the RdRp-CP region of PFYMV has been reported; this sequence shares 84.79% and 95.24% identities at the nucleotide and amino acid levels with isolate PFYMV_Antioquia. Finally, RT-qPCR and Sanger sequencing using specific primers confirmed the presence of PFYMV in different purple passion fruit crops in Antioquia. The present work is the first report of a complete genome sequence of PFYMV.

Palabras clave : Next-generation sequencing; Passifloraceae; Tymovirus; Viral genomes.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )