Resumen

URIBE DELGADO, Nelson; SIERRA BALCARCEL, Raúl Fernando  y  ESPINOSA GONZALEZ, Cindy Tatiana. Comparison of the technique Kato-Kazt, TSET and TSR in diagnosis of the infection by Fasciola hepatica in humans.. Rev. Univ. Ind. Santander. Salud [online]. 2012, vol.44, n.3, pp.7-12. ISSN 0121-0807.

Introduction: fasciolosis is a disease caused by the parasite Fasciola hepatica wich primarily affects cattle, sheep, and goats. In certain areas, it is endemic for human beings. In clinical and epidemiological research of human fasciolosis is important to get diagnostic techniques with high sensibility and specificity. Objective: perform the comparison between three coprological techniques for the diagnosis of infection by F. hepatica in humans. Methods: 200 grams of human feces were experimentally contaminated with F. hepatica eggs obtained from previous analysis with cattle and sheep faecal samples. The preparation was divided into 10 equal parts, each one was processed by the Kato- Katz technique, the spontaneous tube sedimentation technique (STST) and rapid sedimentation technique (TSR). Finally, all samples reading was based on the double-blind study design. Results: we found out that TSR was the most sensitive of the three techniques tested: 7,5 of 10 samples analyzed were positive, followed by the Kato-Katz technique with 4.5 of 10 samples and TSET with only one of 10 samples. Conclusion: This study concludes that the rapid sedimentation technique is the tool of choice for professionals because it is simple, inexpensive and easily applied in laboratories with levels complexity one and two, for a successful diagnosis of human fasciolosis. Salud UIS 2012; 44 (3): 7-12

Palabras clave : Fasciolosis diagnosis by coprology; Kato Katz; technique of spontaneous sedimentation in tube; rapid sedimentation technique.

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