Resumen

GONZALEZ ORTIZ, Fredy Andrés; TORRENEGRA ESCORCIA, Daniel  y  ALBIS ARRIETA, Alberto Ricardo. Biosynthesis of silver nanoparticles with Chlorella sp. Rev. ion [online]. 2021, vol.34, n.2, pp.7-15.  Epub 27-Jul-2021. ISSN 0120-100X.  https://doi.org/10.18273/revion.v34n2-2021001.

In this work, we show the results of the biosynthesis of silver nanoparticles using the microalga Chlorella sp. cultured in several media containing glycerol concentrations ranging between 5% and 20%, and varying light and temperature conditions. Nanoparticle synthesis was performed using supernatants and pellets of autotrophic, heterotrophic, and mixotrophic microalgae cultures. The presence of silver nanoparticles was verified by ultraviolet - visible spectroscopy, and samples that show the highest nanoparticle concentrations were characterized using scanning electronic microscopy. Mixotrophic conditions enhanced the excretion of exo-polymers that improved silver reduction and nanoparticle synthesis. The obtained nanoparticles showed cylindrical shape with dimensions of 108 nm x 156 nm and 87 nm x 123 nm for synthesis undertaken with supernatants of mixotrophic cultures with glycerol 5% and 10%, respectively.

Palabras clave : Green Chemistry; Silver Nanoparticles; Biosynthesis; Microalga.

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