Resumen

MORENO-GALVAN, Andrés; ROJAS-TAPIAS, Daniel F.  y  BONILLA, Ruth. Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs. Corpoica cienc. tecnol. agropecu. [online]. 2012, vol.13, n.2, pp.201-206. ISSN 0122-8706.

For mass plant growth-promoting inoculant production, a high-yield culture medium is fundamental. Sequential application of statistical designs was used to optimize Azospirillum brasilense C16 biomass production. Six nutritional (glycerol, glutamate, mannitol, citric acid, yeast extract and K₂HPO₄ 3H₂O) and three mineral sources (MgSO₄ 7H₂O, FeCl₃ and NaCl) were evaluated using five statistical experiments Placket-Burman, factorial design, steepest ascent, response surface analysis, and mineral screening. The optimum medium composition (g L^-1) was as follows: 28.33 glutamate, 2.92 yeast extract, 1.34 K₂HPO₄ 3H₂O, 0.5 MgSO₄ 7H₂O and 0.02 FeCl₃. After 24 hours of incubation, protein (32.04 mg) and dry biomass (1.51 g L^-1) were 1.72 and 1.68 times higher than in conventional growth medium.

Palabras clave : culture medium optimization; factorial designs; culture medium design; bioinoculant production.

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