Resumo

JURADO-GAMEZ, HENRY; MARTINEZ B, JAVIER ANDRÉS; CHASPUENGAL T, AURA MAGDALENA  e  CALPA Y, FREDY YESID. EVALUATION in vitro OF THE ACTION OF Lactobacillus plantarum WITH PROBIOTIC CHARACTERISTICS ON Yersinia pseudotuberculosis. Rev.Bio.Agro [online]. 2014, vol.12, n.2, pp.49-59. ISSN 1692-3561.

Withtheobjectivetocheckthepotential probiotic of Lactobacillusplantarumonone of themostfrequentdiseases in guinea pig(Cavia porcellus)causedbyYersiniapseudotuberculosis, testswereconducted of inhibition and comparisonin front of antibiotics, showing favorable results, theinhibittothepathogenicbacterium. Itvaluedby HPLC thepossiblepeptidesproducedbyL. plantarum,characterizinganpresence of twopeptides. Tests were performed for the selection of lactic bacteria which showed the following profile: catalase-negative, not producer of gas, resistant to bile salts (0, 5%, 1%, 2% and 3%), pH (2,5, 3,5 and 7,6) and temperature of 38 at 45°C. Also determined the characteristics of the kinetics of fermentation, using two mediums, where it is evaluated CFU/mL, pH, consumption of total sugars (mg/L) and production of lactic acid (%); Lactobacillus plantarum reached the exponential phase of growth in the mediums MRS and Pro, at the 12 and 14:24 hours, with values of 7,0x1011UFC/mL and 5,0x1013UFC/mL, respectively. A design of blocks was applied at random with two treatments and eleven blocks, the result allowed to determine that it doesn't exist significant statistical differences (P>0,05) between the proposed means, but if between the hours of evaluation (P <0,05).

Palavras-chave : Probióticos; Bacteria patógena; Inhibición; Bacteria láctica; Cinética de fermentación; Probiotics; Bacteria pathogen; Inhibition; Lactic bacteria; Kinetics of fermentation.

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