Resumo

SUAREZ-CONTRERAS, LILIANA-YANET,  e  PENARANDA-FIGUEREDO, FREDDY-ARMANDO,. Molecular identification of filamental fungi and its biotechnological potential. Rev.Bio.Agro [online]. 2022, vol.20, n.1, pp.194-206.  Epub 30-Dez-2021. ISSN 1692-3561.  https://doi.org/10.18684/bsaa.v20.n1.2022.1914.

Filamentous fungi are organisms frequently used to develop alternatives to solve challenges in different productive representing a competitive opportunity with economic value in the agriculture, food and pharmaceutical, as well as industrial and biofuels sector Continuing previous research at Universidad Francisco de Paula Santander (UFPS), 25 samples from the strain bank were used for molecular characterization and taxonomic identification. DNA from each strain was obtained under the phenol-chloroform procedure, then, using PCR technique and internal transcript spacer (ITS) primer 4 and 5, amplicons were acquired to be sequenced. Results showed taxonomy categorizations in 4 orders Hypocreales, Eurotiales, Pleosporales y Saccharomycetales with specimens from genus like Aspergillus, Curvularia; Purpureocillium, Penicillium y Trichoderma among others. Findings were laid into a cladogram to display their phylogenetic proximity, and connecting their biotechnological potential towards developing bio-materials, pharmaceutical products and bio-catalysts. Thanks to present study and data base review, it was possible to advance into describing the biological capabilities of these fungi towards developing biotechnological products and services with an approach in phytopathology research.

Palavras-chave : DNA; Bioprospecting; Cladogram; Biologic control; Homology; ITS; Molecular marker; Molds; Pathogen; PCR.

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