Resumo

FAJARDO-ARGOTI, IVONNE-CATALINA,; JURADO-GAMEZ, HENRY-ARMANDO,  e  PARRA-SUESCUN, JAIME-EDUARDO,. Probiotic viability and inhibitory capacity of microencapsulated L. gasseri on L. monocytogenes and S. aureus. Rev.Bio.Agro [online]. 2022, vol.20, n.2, pp.87-101.  Epub 01-Jul-2022. ISSN 1692-3561.  https://doi.org/10.18684/rbsaa.v20.n2.2022.2042.

Lactobacillus gasseri is recognized as an important commensal microorganism, capable of inhibiting multiple pathogens through the production of antimicrobial substances and by its influence on the mechanisms of the immune system at the intestinal level. Listeria monocytogenes and Staphylococcus aureus, on the other hand, are potentially harmful zoonotic microorganisms that affect animal and human health and, in turn, are contaminants of the food chain. In this context, the effect of microencapsulated L. gasseri on L. monocytogenes and S. aureus was evaluated. Bacterial growth of the lactic strain was determined in two culture media (MRS-PRO), microencapsulated in a 15 % inulin-maltodextrin matrix. Viability was evaluated over time and at in vitro gastrointestinal conditions at 8,15, 20, 20, 30 and 45 days with maximum values of 3,3 x 10⁹ CFU/mL. Then, capsule morphology was identified by scanning electron microscopy (SEM) with capsule sizes between 2,69 and 12,26 µm. Finally, the minimum inhibitory concentration (MIC) was established to inhibit the pathogens. Optimal viabilities of the lactic strain were observed in the different conditions evaluated, the encapsulating matrix showed effectiveness in the protection of the strain, two of the proposed methods showed greater inhibition capacity against the pathogenic strains. L. gasseri is an acceptable and safe strain for its application for the benefit of animal and human health.

Palavras-chave : Encapsulating matrix; Thermoprotectant; Viability; Inhibition; Probiotic; Probiotic, Prebiotic; Animal and human health; Food safety; Foodborne toxicity; Antimicrobial resistance.

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