Resumo

RIANO A, Luz Dary  e  ZAMORA E, Humberto Miguel. ANTIFUNGAL PROTEINS AGAINST P. INFESTANS FROM INTRACELLULAR SPACE OF TOMATO LEAVES (Lycopersicon esculentum cerasiforme) POSSIBLE DEFENSINS OF PLANTS. Rev.Colomb.Quim. [online]. 2005, vol.34, n.1, pp.7-23. ISSN 0120-2804.

Antifungal Proteins from intercellular space of Tomato leaves Lycopersicon esculentum cerasiforme were isolated and partially characterized; this variety had shown resistance against Phytophthora infestans, after inoculating plants with the pathogen it was observed that these proteins were accumulated systemically. The isolated proteins be haved as plant defensins, a novel protein family, with low molecular weight, cationics at physiological pH and Antifungal Activity in vitro evaluated against P. infestans. Electrophoretic analyses in SDS Tricine-PAGE, under reducing and non-reducing conditions suggested that, they were associated as trimers and tetramers; and have molecular weights of 5,2 kDa.

Palavras-chave : Intercellular space; leaves; tomato; PR (pathogenesis related) proteins; Plant Defensins; Antifungal activity.

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