Resumo

VERJAN - GARCIA, Noel; IREGUI - CASTRO, Carlos A.  e  HIRONO, Ikuo. A random genome analysis of Edwardsiella tarda ETSJ54: annotation of putative virulence - related genes. Orinoquia [online]. 2013, vol.17, n.1, pp.69-83. ISSN 0121-3709.

As an initial step to understand the pathogenic mechanisms displayed by Edwardsiella tarda during infection in fish, we conducted a random genome sequencing of cosmid and plasmid DNA libraries generated from a virulent E. tarda strain (ETSJ54) to identify putative virulence-related genes. The assumed virulence-related genes of E. tarda were grouped into nine categories including chemotaxis and motility, adhesion and invasion, endotoxin (LPS), toxin secretion by type I and type III secretion systems, iron uptake, proteases, and intra-macrophage survival. The results reveal that E. tarda is equipped with a wide range of genes involved in virulence and pathogenesis of diverse bacterial genera and species including Salmonella, Yersinia and Vibrios species. The results also indicate a high genetic flux in the E. tarda genome that could explain in some extent its potential to infect and to cause disease in a number of animal species.

Palavras-chave : Edwardsiellosis; genome sequencing; virulence; pathogenesis.

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