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Revista MVZ Córdoba
versão impressa ISSN 0122-0268versão On-line ISSN 1909-0544
Resumo
CORREA-VALENCIA, Nathalia; MOYANO, Roberto Damián; ROMANO, María Isabel e FERNANDEZ-SILVA, Jorge A. Tipificación molecular de Mycobacterium avium subsp. paratuberculosis en lecherías de Antioquia, Colombia. Rev.MVZ Cordoba [online]. 2020, vol.25, n.3, pp.73-79. Epub 04-Ago-2022. ISSN 0122-0268. https://doi.org/10.21897/rmvz.1816.
Objective.
To determine Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in environmental samples from Colombian dairy herds.
Materials and methods.
Environmental samples from 25 IS900-qPCR MAP-positive dairy herds were cultured by duplicate in Herrold's egg yolk medium with mycobactin J to obtain isolates. Suspicious colonies were confirmed by MAP-IS900-qPCR. Positive DNA was sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) and multilocus short sequence repeats (MLSSR) techniques to analyze the genetic differences between the isolates.
Results.
Sub-typing revealed two different genotypes by MIRU-VNTR (INMV 2 and INMV 36). MLSSR technique was carried out to increase the discriminatory power from what was obtained by MIRU-VNTR, but no differences were observed among the recovered isolates.
Conclusions.
The present study represents an important approach to the knowledge on MAP epidemiological status in the study population.
Palavras-chave : Genetics; genotyping; Johne's disease; MLSSR; MIRU-VNTR (Source: National Agricultural Library Thesaurus).
