Resumo

JEREZ-MOGOLLON, Silvia-Juliana et al. IMPROVEMENT OF LAB-SCALE PRODUCTION OF MICROALGAL CARBOHYDRATES FOR BIOFUEL PRODUCTION. C.T.F Cienc. Tecnol. Futuro [online]. 2012, vol.5, n.1, pp.103-116. ISSN 0122-5383.

ABSTRACT This work studied the improvement of biomass and carbohydrate (glucose and xylose) lab-scale productivity in Chlorella vulgaris UTEX 1803 through the use of the carbon/nitrogen ratio. In order to do so, mixotrophic cultures were made by the modification of initial concentration of CH₃COONa (5, 10 and 20 mM) and NaNO₃(0.97, 1.94 and 2.94 mM). All treatments were maintained at 23 ± 1°C, with light/dark cycles of 12h : 12h for 5 days. It was found that in addition to the carbon/nitrogen ratio, time also influences the concentration of biomass and carbohydrates. The treatment containing 10 mM acetate: 1.94 mM nitrate, reached a concentration of 0.79 g/L of biomass, 76.9 µg/mL of xylose and 73.7 µg/mL of glucose in the fifth day. However, the treatment containing 20 mM acetate: 0.97 mM nitrate produced 1.04 g/L of biomass, 78.9 µg/mL of xylose and 77.2 µg/mL of glucose in the third day, while in the same day the treatment containing 0 mM acetate: 2.94 mM nitrate, produced 0.55 g/L of biomass, 40.2 µg/mL of xylose and 31.3 µg/mL of glucose. The use of carbon/nitrogen ratios improved biomass productivity (from 0.55 to 1.04 g/L) as well as xylose (from 40.2 to 78.9 µg/mL) and glucose (from 31.3 to 77.2 µg/mL) concentration, representing an improvement of up to two times the production of both biomass and carbohydrates in only 3 days of culture.

Palavras-chave : Chlorella vulgaris; Mixotrophic culture; Glucose; Xylose; Microalgae; Biomass; Sodium acetate treatment; Sodium nitrate treatment.

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