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Nova
versão impressa ISSN 1794-2470
Resumo
LAURA KATERINE, Parada-Ferro; GUALTEROS-BUSTOS, Andrea Viviana e SANCHEZ-MORA, Ruth Mélida. Phenotypic characterization of the N2 strain of Caenorhabditis elegans as a model in neurodegenerative diseases. Nova [online]. 2017, vol.15, n.28, pp.69-78. ISSN 1794-2470.
The nematode C. elegans was established since I960, thanks to the South African biologist Sydney Brenner, as a model organism in research. Their biological qualities allow to improve the vision and understanding of pathologies in human and other multicellular beings; In addition, its clear and observable phenotypes make it a suitable organism for the basic study of neurodegenerative, immunological diseases and carcinogenic processes.
Objective.
To analyze the phenotypic characteristics of the C. elegans N2 wild strain for later use as a screening model in the Biotechnology and Genetics Laboratory (Colegio Mayor de Cundinamarca University).
Materials and Methods.
The nematode was grown and grown in the NGM medium with the strain E. coli OP50. The N2 strain was synchronized to obtain eggs and later L1 larvae. The tests of longevity, reproduction, length and thermal stress were standardized.
Results.
The phenotypic characterization of the N2 strain of C. elegans presented a longevity of 16 to 22 days, an average reproduction of 225 offspring, the length of the nematode was 1100 ± 50 μm and the survival under thermal stress evaluated in the two Development stages of the nematode is greatly reduced at 37 ° C compared to 35 ° C; In addition, nematodes were more resistant to the first day of young adult compared to the sixth day of adulthood.
Conclusions.
The results of this study suggest that the phenotypic characteristics of the nematode analyzed are within the literature, so it is feasible to use it as a biological model in different trials as reported in other studies.
Palavras-chave : C. elegans; Neurodegenerative; Longevity; Reproduction; Larval stages..
