Resumo

OSORIO, José Henry  e  FLOREZ OCHOA, Jancy Darly. Comparison between the polyvinyl sulphate precipitate method and the Detergents + DSBmT direct method to determine LDL cholesterol in lying hens. Ces. Med. Vet. Zootec. [online]. 2013, vol.8, n.1, pp.61-69. ISSN 1900-9607.

Summary The objective of this study was to compare two methods (precipitate vs. direct) to quantify low-density lipoprotein cholesterol in laying hens. Materials and methods: Cholesterol in low density lipoproteins was quantified using the precipitate (polyvinyl sulfate) method and the direct method based on detergent and N,Nbis (4-sulfobutyl )-m-toluidine. A total of 40 laying hens (Hy -Line W-36) aged 26 weeks were used. Results: Cholesterol levels (mg/dL) assessed by the precipitate and direct methods were 117.3 ± 38.6 and 49.3 ± 11.1, respectively. The P value for the F test was less than 0.05, showing statistically significant difference between methods, with a 95% confidence. Conclusions: The direct method based on detergents and N,Nbis(4-sulfobutyl)-m-toluidine can be used to determine cholesterol in low density lipoproteins of laying hens since it is not affected by serum triglyceride levels.

Palavras-chave : Detergent; DSBmT; LDL- cholesterol; polyvinyl sulfate.

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