Resumo

RESTREPO BETANCUR, Giovanni; USUGA SUAREZ, Alexandra  e  ALBERTO ROJANO, Benjamín. Techniques for analyzing the potential fertility of stallion semen. Ces. Med. Vet. Zootec. [online]. 2013, vol.8, n.1, pp.115-127. ISSN 1900-9607.

In order to optimize the results of assisted reproductive procedures such as artificial insemination, the potential fertility of stallion semen should be determined. However, conventional processes of seminal evaluation can give an low predictive value of the pregnancy rate. For this reason, the new methods of semen analysis incorporate several technological resources and improve the existing techniques. In many cases those techniques have been adapted to the equine species. The development of the computer-assisted semen analysis (CASA) has allowed a more objective and accurate sperm evaluation, including the identification of new variables with diagnostic value. The finding of a wide variety of fluorochromes and compounds conjugated with fluorescent probes, and the development of various technologies to visualize and quantify the fluorescence emmited by the cell and cell compartments allows a more complete sperm analysis. The application of tests or techniques using oocytes from other species or even parts of the zona pellucida has favored a more accurate diagnosis of the true sperm fertilizing capacity in horses. The aim of this review is to provide and analyze information on recent conventional methods used to assess the potential fertility of stallion semen.

Palavras-chave : CASA; evaluation seminal; fertilizing capacity; fluorescence.

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