Resumo

HERNANDEZ HERRERA, Darwin Yovanny; MUNOZ FLOREZ, Jaime Eduardo  e  ALVAREZ FRANCO, Luz ángela. Genetic diversity of BoLA-DRB3 gene in Colombian creole Hartón del Valle cattle. Ces. Med. Vet. Zootec. [online]. 2015, vol.10, n.1, pp.18-30. ISSN 1900-9607.

The objective of this study was to evaluate the genetic diversity of ball-DRB3 gene in the Colombian creole Hartón del Valle cattle. A total of 93 Hartón del Valle (HV), 30 Lucerne (LUC), and 30 Holstein (HOL) animals were evaluated for DRB3 gene polymorphism using the PCR-SBT method. The number of alleles found were 27, 17 and 14 for HV, LUC, HOL, respectively. The most frequent alleles were DRB3*1101 (14.5%) in HV; LUC DRB3 * 0701 in LUC; and *1107, *1501 and *2301 in HOL (11.7%). Expected heterozygosity values were higher than observed, which resulted in a low fixing index, positive F _IS (significant only for HV), and no significant deviations from Hardy- Weinberg equilibrium for LUC. The molecular analysis of variance reflected little variation between breeds (3%) and a moderate genetic differentiation (F _ST = 0.056 p<0.01). Regarding sequence, LUC was the most diverse breed, no neutral selection is occurring, and none of the breeds is in selection equilibrium. Comparison with reports on other breeds showed high genetic diversity in Colombian breeds. We conclude that HV has high genetic diversity in BoLA-DRB3 locus. The cause of this polymorphism may be due in part to the origin of HV. This high variation can be maintained by selection equilibrium.

Palavras-chave : Genetic marker; major histocompatibility complex; PCR-SBT.

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